食品科学2011，Vol.32Issue(16)：245-248,4.
高效液相色谱法测定食品中的安赛蜜
Determination of Acesulfame K in Food by High Performance Liquid Chromatography
白静1
作者信息
- 1. 北京市通州区产品质量监督检验所,北京101100
- 折叠
摘要
Abstract
A high performance liquid chromatography method for the determination of the artificial sweetener acesulfame K in food was developed.Ultrasonic-assisted aqueous extraction followed by the removal of protein impurities by adding zinc acetate and potassium ferrocyanide was used for sample preparation.Acesulfame K was separated on a C18 column using methanol-0.02 mol/L ammonium acetate（5.5：94.5,V/V） as mobile phase at a flow rate of 0.8 mL/min and detected using a diode array detector（DAD） at 230 nm.The column temperature was set at 30 ℃ and the injection volume 5μL.The retention time of acesulfame K was about 4.6 min.The detection limit of the developed method was 1.7 mg/kg,and the linear range was between 1.0 and 50.0μg/mL.The mean spike recoveries for acesulfame K in a blank juice sample exceeded 90%,with a relative standard deviation below 2%（n=6）.In this method,acesulfame K was extracted using water as solvent with the assistance of ultrasound,and purified by precipitation.The method proved to be simple,rapid,accurate and practical.关键词
安赛蜜/高效液相色谱/测定Key words
acesulfame K/high performance liquid chromatography（HPLC）/determination分类
轻工纺织引用本文复制引用
白静..高效液相色谱法测定食品中的安赛蜜[J].食品科学,2011,32(16):245-248,4.
