食品科学2011,Vol.32Issue(16):290-294,5.
多维数据分析法同时分辨3种荧光光谱重叠的氨基酸
Simultaneous Detection of 3 Amino Acids with Overlapped Fluorescent Spectra by Multi-dimensional Data Analysis
摘要
Abstract
An orthogonal signal correction-wavelet packet transform-partial least squares(OSC-WPT-PLS) method was proposed for simultaneous detection of tryptophan,tyrosine and phenylalanine with overlapped fluorescent spectra.A program(POSCWPTPLS) was designed to execute relevant calculations and three chemometric methods(OSC-WPT-PLS,WPT-PLS and PLS) were compared in this study.The relative standard errors of prediction(RSEP) were achieved for all components by using OSC-WPT-PLS,WPT-PLS and PLS were 2.80%,4.35% and 5.14%,respectively.The OSC-WPT-PLS method revealed the best detection efficiency among three methods,which could be successfully applied to determine the contents of tryptophan,tyrosine and phenylalanine in water,Gold-Camel wine and Hetao wine made in Inner Mongolia with a recovery rate of respectively 96.9%-103.2%,94.1%-105.4% and 95.3%-107.8%.关键词
多维数据分析/荧光光度法/同时测定/色氨酸/酪氨酸/苯丙氨酸Key words
multi-dimensional data analysis/fluorespectrophotometry/simultaneous determination/tryptophan/tyrosine/phenylalanine分类
化学化工引用本文复制引用
薛永林,任守信,张雁冰,高玲..多维数据分析法同时分辨3种荧光光谱重叠的氨基酸[J].食品科学,2011,32(16):290-294,5.基金项目
国家自然科学基金地区项目(21067006 ()
0762003) ()
内蒙古自然科学基金项目 ()