食品科学2011，Vol.32Issue(16)：322-325,4.
微波消解-释放剂辅助火焰原子吸收分光光度法测定蛋白质粉中的钙
Determination of Calcium in Protein Powder by Microwave Digestion-FAAS with Releasing Agent
摘要
Abstract
A method has been established for the determination of calcium in protein power using microwave digestion-flame atomic absorption spectrophotometry with La（NO3）3 as the releasing agent.Digestion efficiency was investigated with respect to four variables including digestion system,digestion pressure,digestion time and pressurization style.The effect of La（NO3）3 concentration on the release of calcium was discussed.Under optimal conditions,the linear range of this method for calcium was 1-20μg/mL and the detection limit was 0.15μg/mL.The average calcium content in a commercial protein powder sample determined by this method was 0.46% with a relative standard deviation of 1.63%（n = 6）.The average recovery rate of calcium in a protein powder sample with a calcium content of 9.10μg/mL across the spike levels of 0.5,1.5μg/mL and 2.0μg/mL was 96.8% with a relative standard deviation of 2.34%.Therefore,this method has a wide linear dynamic range,good correlation,high accuracy and precision,and high practical application value.关键词
微波消解/释放剂/火焰原子吸收分光光度法（flame/atomic/absorption/spectrophotometry,FAAS）/蛋白质粉/钙Key words
microwave digestion/releasing agent/flame atomic absorption spectrophotometry（FAAS）/protein powder/calcium分类
化学化工引用本文复制引用
刘全德,唐仕荣,陈尚龙..微波消解-释放剂辅助火焰原子吸收分光光度法测定蛋白质粉中的钙[J].食品科学,2011,32(16):322-325,4.基金项目
江苏省高校自然科学基金项目 （）
