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杜仲翅果油体外抗氧化能力研究

向志钢 李先辉 张永康

食品科学2011,Vol.32Issue(17):133-136,4.
食品科学2011,Vol.32Issue(17):133-136,4.

杜仲翅果油体外抗氧化能力研究

Antioxidant Activity in vitro of Seed Oil from Eucommia ulmoides Oliver Key Fruits

向志钢 1李先辉 2张永康1

作者信息

  • 1. 吉首大学医学院,湖南吉首416000
  • 2. 吉首大学植物资源保护与利用湖南省高校重点实验室,湖南吉首416000
  • 折叠

摘要

Abstract

Objective: To study the antioxidant activity of seed oil from Eucommia ulmoides Oliver key fruits in vitro.Methods: The reducing power and superoxide anion radical scavenging rate of the oil were measured by trichloroacetic acid method and NBT photoreduction method,respectively.The scavenging rate against hydroxyl radicals generated in Fenton system was determined by NBT photoreduction method.The cell membrane-protecting effect was determined by oxidative hemolysis of erythrocytes and the inhibitory effect against lipid peroxidation was evaluated by determining the relative content of MDA in mouse liver homogenate.Vitamin C was used as the control.Results: The oil had strong reducing power so that it could remarkably eliminate superoxide anion free radicals and hydroxyl free radicals.In addition,the oil could also inhibit the generation of MDA in mouse liver homogenate,reduce H2O2-mediated oxidation hemolysis of erythrocytes and spontaneous lipid peroxidation of mouse liver tissue in a dose-dependent manner.The oil at the concentration of 9 mg/mL revealed the strongest effect.Conclusion: Seed oil from Eucommia ulmoides Oliver key fruits has antioxidant capability in vitro.

关键词

杜仲翅果油/抗氧化性/自由基

Key words

seed oil from Eucommia ulmoides Oliver key fruits/antioxidant capability/free radicals

分类

医药卫生

引用本文复制引用

向志钢,李先辉,张永康..杜仲翅果油体外抗氧化能力研究[J].食品科学,2011,32(17):133-136,4.

基金项目

植物资源与利用湖南省高校重点实验室开放基金课题 ()

食品科学

OA北大核心CSCDCSTPCD

1002-6630

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