食品科学2011，Vol.32Issue(17)：250-253,4.
副干酪乳杆菌乳酸脱氢酶的生物信息学分析
Bioinformatic Analysis of Genes Encoding Lactate Dehydrogenase from Lactobacillus paracasei
摘要
Abstract
Objective： To analyze and predict the structure and characteristics of lactate dehydrogenase from Lactobacillus paracasei for guiding experimental studies of its biological functions and application.Methods： Bioinformatic tools provided by the NCBI website and ExPasy as well as bioinformatic software packages such as ClustalX and VMD were used to identify sequence encoding LDH protein from database.The characteristics including physical-chemical characteristics,post-translational modification sites,topological structure,secondary structures,tertiary structure and domains of the deduced protein were analyzed.The sequences encoding LDH from Lactobacillus paracasei and other microorganisms were compared to obtain the conserved sequence.Results： The gene codes for 335 amino acids were achieved.The theoretical molecular weight of the deduced protein was 36608.8 u.The encoding protein was elucidated to have 3 trans-membrane regions.Through the comparison of amino acid sequences,LDH from Lactobacillus paracasei had high homology with LDH from Lactobacillus casei.Conclusion The structure of ldh gene and LDH protein from Lactobacillus paracasei can be bioinformatically analyzed and predicted.关键词
副干酪乳杆菌/乳酸脱氢酶/生物信息学Key words
Lactobacillus paracasei/lactate dehydrogenase/bioinformatics分类
生物科学引用本文复制引用
赵婷,姚雯轶,王立梅..副干酪乳杆菌乳酸脱氢酶的生物信息学分析[J].食品科学,2011,32(17):250-253,4.基金项目
江苏省科学技术厅农业科技支撑计划项目 （）
常熟市科学技术局农业科技攻关项目 （）
