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产木聚糖酶但无纤维素酶活性菌株的筛选及初步鉴定

陈娜 生吉萍 申琳

食品科学2011,Vol.32Issue(17):273-277,5.
食品科学2011,Vol.32Issue(17):273-277,5.

产木聚糖酶但无纤维素酶活性菌株的筛选及初步鉴定

Isolation and Preliminarily Identification of Cellulase-free Xylanase-producing Strain

陈娜 1生吉萍 1申琳1

作者信息

  • 1. 中国农业大学食品科学与营养工程学院,北京100083
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摘要

Abstract

In order to obtain a strain with cellulase-free xylanase activity,a bacterium named as 26T was isolated from paper mill wastewater by substrate screening.The strain 26T was preliminarily identified as Bacillus subtilis by analyzing its physiological and biochemical characteristics and 16S rDNA gene sequence.This strain could grow under the conditions of 50 ℃ and pH 10,and revealed high heat and alkali resistance.The xylanase from it as an extracellular enzyme had an activity of 14.58 U/mL under the optimal fermentation conditions of 37 ℃,200 r/min and 12 h.Therefore,this strain might have a prosperous application in food industry.

关键词

芽孢杆菌/木聚糖酶/纤维素酶/耐热性/耐碱性

Key words

Bacillus subtilis/xylanase/cellulase/heat resistance/alkali resistance

分类

轻工纺织

引用本文复制引用

陈娜,生吉萍,申琳..产木聚糖酶但无纤维素酶活性菌株的筛选及初步鉴定[J].食品科学,2011,32(17):273-277,5.

基金项目

国家公益性行业(农业)科研专项 ()

食品科学

OA北大核心CSCDCSTPCD

1002-6630

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