食品科学2011，Vol.32Issue(17)：371-375,5.
花生过敏原蛋白分离纯化方法研究进展
Purification Methods of Peanut Allergens：A Review
摘要
Abstract
Currently,11 proteins,including Ara h 1,Ara h 2,Ara h 3,Ara h 4,Ara h 5,Ara h 6,Ara h 7,Ara h 8,Ara h 9,Ara h 10 and Ara h 11,have been identified to be peanut allergens.Recent advances in the research on separation and purification methods for major（Ara h 1,Ara h 2,Ara h 3/4 and Ara h 6） and non-major（Ara h 7-11） peanut allergens,such as ammonium sulfate precipitation,column chromatography and electrophoresis,are introduced in detail in this article.Ammonium sulfate precipitation has been generally used in the rough purification step and column chromatographic methods in the refinement step including ion exchange chromatography,gel filtration chromatography,affinity chromatography,hydrophobic interaction chromatography and high performance liquid chromatography.At present,the most extensively used method for separating and purifying peanut allergens are ion exchange chromatography and gel filtration chromatography.However,electrophoresis is only used to purify Ara h 7 and peanut oleosins（Ara h 10 and Ara h 11）.关键词
花生过敏原/硫酸铵沉淀/柱层析/分离纯化Key words
peanut allergen/ammonium sulfate precipitation/chromatography/purification分类
轻工纺织引用本文复制引用
隗啸南,高金燕,李欣,闫飞,朱江,陈红兵..花生过敏原蛋白分离纯化方法研究进展[J].食品科学,2011,32(17):371-375,5.基金项目
教育部新世纪优秀人才支持计划项目 （）
国家自然科学基金项目 （）
南昌大学食品科学与技术国家重点实验室目标导向项目 （）
