食品科学2011，Vol.32Issue(18)：30-33,4.
大麦虫水溶蛋白的分离纯化及抗氧化性研究
Isolation,Purification and Antioxidant Activity Evaluation of Water Soluble Protein from Giant Mealworm Beetle（Zophobas morio） Larvae
张建新 1郭倩 1何桂梅 1刘娜1
作者信息
- 1. 西北农林科技大学食品科学与工程学院,陕西杨凌712100
- 折叠
摘要
Abstract
Objective： To isolate and purify water soluble protein from giant mealworm beetle（Zophobas morio） larvae and evaluate its antioxidant activity.Methods： The water soluble protein in giant mealworm beetle larvae was extracted with pH 7.4 phosphate buffer,purified by ammonium sulfate precipitation,and fractionated by Sephadex G-100 column chromatography to obtain fractions P1,P2 and P3.Fraction P2 was further fractionated by DEAE-Sepharose FF column chromatography into subfractions P2M1,P2M2 and P2M3.The antioxidant activities of each fraction and each subfraction were investigated by DPPH and hydroxyl free radical scavenging assays.Results： Subfraction P2M2 presented the highest DPPH and hydroxyl free radical scavenging rates,which were 99.42% and 55.11%,respectively.关键词
大麦虫/蛋白/分离纯化/抗氧化Key words
giant mealworm beetle/protein/isolation and purification/antioxidant activity分类
化学化工引用本文复制引用
张建新,郭倩,何桂梅,刘娜..大麦虫水溶蛋白的分离纯化及抗氧化性研究[J].食品科学,2011,32(18):30-33,4.
