食品科学2011，Vol.32Issue(18)：142-147,6.
南极磷虾酶解工艺优化及模型建立
Optimization and Modeling of Enzymatic Hydrolysis Process for Antarctic Krill
摘要
Abstract
In the present work,alcalase was identified as the most suitable enzyme for enzymatic hydrolysis of Antarctic krill among 7 commonly used enzymes based on simultaneous consideration of chloroacetic acid-nitrogen soluble index（TCA-NSI） and degree of hydrolysis（DH）.In order to optimize the hydrolysis of Antarctic krill by alcalase,the effects of the hydrolysis conditions enzyme dosage,substrate concentration,pH,temperature and hydrolysis time on TCA-NSI and DH were studied by one-factor-at-a-time and orthogonal rotary composite design methods.Two regression models with TCA-NSI or DH as a function of each hydrolysis condition were established.The optimal process conditions for hydrolyzing Antarctic krill with alcalase were 50.7 ℃ hydrolysis temperature,pH 8.01,3010 U/g alcalase dosage and 239 min hydrolysis time.Under the optimal conditions,the TCA-NSI and DH were 73.02% and 42.33%,respectively.Meanwhile,peptides with an average length of 2.36 and a molecular mass of 277.9 were obtained.关键词
南极磷虾/Alcalase/短肽/水解度Key words
Antarctic krill/alcalase/oligopeptide/hydrolysis degree分类
轻工纺织引用本文复制引用
吕传萍,李学英,杨宪时,郭全友..南极磷虾酶解工艺优化及模型建立[J].食品科学,2011,32(18):142-147,6.基金项目
国家“863”计划项目 （）
中央级公益性科研院所基本科研业务费专项 （）
