食品科学2011，Vol.32Issue(18)：234-238,5.
催化光度法测定三聚氰胺脱氨酶活性
Determination of Melamine Deaminase Activity by Catalytic Spectrophotometry
季艳伟 1许杨 2黄志兵 1涂追2
作者信息
- 1. 南昌大学食品科学与技术国家重点实验室
- 2. 中德联合研究院,江西南昌330047
- 折叠
摘要
Abstract
A method to determine melamine deaminase activity by catalytic spectrophotometry was developed based on Berthelot colour reaction.The melamine deaminase activity was calculated by the amount of ammonia released per minute.The reaction conditions for melamine deaminase were optimized by one-factor-at-a-time experiments combined with an orthogonal experiment design L9（34）.Optimum coloration reaction condition was obtained when the reaction was allowed to proceed for 40 min in a system composed of 0.3 mL of sodium hypochlorite and 1.2 mL of salicylic acid solution and detection at a wavelength of 650 nm.The optimal enzymatic reaction conditions was found to be 0.2 mL melamine solution,60 μL crude enzyme and 40 min reaction time.Under the optimized conditions,the melamine deaminase activity was 132.41 U/L with inter-assay and intra-assay coefficients of variation of 0.81%-1.19% and 1.27%-1.69%,respectively.关键词
三聚氰胺脱氨酶/酶活性测定/分光光度法Key words
melamine deaminase/enzyme activity determination/catalytic spectropotometry分类
生物科学引用本文复制引用
季艳伟,许杨,黄志兵,涂追..催化光度法测定三聚氰胺脱氨酶活性[J].食品科学,2011,32(18):234-238,5.
