食品科学2011，Vol.32Issue(18)：256-259,4.
HPLC法测定不同产地黑豆皮中矢车菊素-3-O-葡萄糖苷的含量
HPLC Determination of Cyanidin-3-O-Glucoside in Black Soybean Hulls Grown in Different Regions of China
摘要
Abstract
Objective： To determine cyanidin-3-O-glucoside（C3G） content in black soybean hulls grown in different regions of China by HPLC.Methods： The chromatographic separation was performed on Phenomenex Luna Su C18（250 mm × 4.60 mm,5 μm） column by gradient elution using 0.5% phosphoric acid solution as mobile phase A and water： acetonitrile（50：50,V/V） as mobile phase B at a flow rate of 0.8 mL/min.The column temperature was set at 30 ℃and the detection wavelength at 520 nm.Results： The developed regression equation of cyanidin-3-O-glucoside standard is： Y = 2 × 107X-33120（r= 0.9998）.A good linearity was shown in the range of 0.1041-1.041 μg.The average recoveries at three spike levels were 92.4%,92.5% and 95.5%,respectively.The contents of cyanidin-3-O-glucoside in samples from Northeast China and Shandong,Hubei and Anhui provinces were in the range of 5.263-12.829 mg/g.Conclusions： The method is simple and reliable,and may be used for the quality control of black soybean hull from different geographic origins.关键词
高效液相色谱法（HPLC）/黑豆皮/矢车菊素-3-O-葡萄糖苷/含量测定Key words
high performance liquid chromatography（HPLC）/black soybean hulls/cyanidin-3-O-glucoside/content determination分类
轻工纺织引用本文复制引用
刘占云,刘晓秋,白淑芳,刘岱琳..HPLC法测定不同产地黑豆皮中矢车菊素-3-O-葡萄糖苷的含量[J].食品科学,2011,32(18):256-259,4.基金项目
天津市科技支撑项目 （）
