食品科学2011，Vol.32Issue(18)：300-303,4.
反相高效液相色谱法同时测定马铃薯块茎中酚酸类物质
Simultaneous Determination of Phenolic Acids in Potato Tuber by RP-HPLC
摘要
Abstract
Objective： A reversed-phase high performance liquid chromatographic（RP-HPLC） method was developed to determine the contents of chlorogenic acid,caffeic acid,p-coumaric acid and ferulic acid in potato tuber from Jiangxi,Beijing and Xinjiang.Methods： The contents of phenolic compounds were measured by RP-HPLC using YMC-C18 column（250 mm × 4.6 mm,5μm） as the sationary phase and CH3OH： H2O： CH3COOH（30：70：1,V/V） as the mobile phase at a flow rate of 1.0 mL/min.Results： A good linear relationship of this method was achieved in the ranges of 7.80-1000μg/mL for chlorogenic acid,0.78-100μg/mL for caffeic acid,0.312-40μg/mL for p-coumaric acid and 0.078-10μg/mL for ferulic acid with a correlation coefficient of 0.9968-0.9999.The average recovery rates of this method were 92.38%-101.73%.Conclusion： The developed RP-HPLC method is characteristic of fast determination,accurate results,high sensitivity and good reproducibility.In addition,different growth environments of potato tuber have an important impact on the contents of phenolic acids.关键词
马铃薯/块茎/酚酸类物质/反相高效液相色谱法Key words
potato/tuber/phenolic compounds/reversed-phase high performance liquid chromatography（RP-HPLC）分类
轻工纺织引用本文复制引用
骆成尧,印遇龙,阮征,欧阳崇学,文红艳,周笑犁,彭彰智..反相高效液相色谱法同时测定马铃薯块茎中酚酸类物质[J].食品科学,2011,32(18):300-303,4.基金项目
南昌大学“赣江学者奖励计划”项目 （）
