食品科学2011，Vol.32Issue(19)：88-91,4.
茶枝柑皮多糖的分离纯化及单糖组成分析
Isolation,Purification and Monosaccharide Composition Analysis of Citus reticulata Peel Polysaccharides
摘要
Abstract
Polysaccharides were extracted from dried powder of Citus reticulata peel with acidic water,followed by pigment removal on D-201 ion exchange resin,protein removal by Sevag,s method,dialysis and freeze-drying.The CPA-I fraction was achieved by sequential column chromatographies on DEAE-sepharose fast flow and Sephadex G-100.Monosaccharide composition analysis of CPA-I was carried out by HPLC based on pre-column derivatization with 1-phenyl-3-methy1-5-pyrazolone（PMP）.High performance size exclusion chromatography（HPSEC） was used for purity analysis and relative molecular weight determination.UV-visible and infrared（IR） spectral analyses were conducted for structural characterization.The results showed that the molecular weight of CPA-I with high purity was 7.96×104,and the monosaccharide composition of the polysaccharide was composed of galacturonic acid and arabinose.The IR spectrum indicated that CPA-I was a galacturonic acid polysaccharide esterified by carboxylic acid.关键词
茶枝柑皮/多糖/分离纯化/单糖组成Key words
Citus reticulata peel/polysaccharide/isolation and purification/monosaccharide composition分类
化学化工引用本文复制引用
陈思,黄庆华,李娆玲,甘伟发,源翰祺..茶枝柑皮多糖的分离纯化及单糖组成分析[J].食品科学,2011,32(19):88-91,4.基金项目
广东省教育厅大学生创新实验项目 （）
