食品科学2011，Vol.32Issue(19)：161-164,4.
高产γ-氨基丁酸富锌乳酸菌的分批及补料发酵研究
Batch and Fed-batch Fermentation of High Yield Gamma-aminobutyric Acid-producing Lactobacillus brevis BS2 Capable of Enriching Zn
摘要
Abstract
In this study,Lactobacillus brevis BS2,a high yield gamma-aminobutyric acid（γ-GABA）-producing strain capable of enriching Zn,was used to conduct batch and fed-batch fermentation in a 15-L fermenter for exploring the biotransformation of γ-GABA.An initial pH of 5 was used for batch fermentation with and without holding pH at 5.As a result of holding pH,the production ofγ-GABA was considerably increased and sodium glutamate and glucose were almost all consumed at the time points of 32 h and 44 h,respectively.When the initial pH of 5 was held during the fermentation,adding 200 mL of 630 g/L sodium glutamate and 200 mL of 550 g/L glucose（18 min needed at the same flow rate of 11.1 mL/min） was necessary at the time points of 32 h and 44 h,respectively.As a result,the glucose and sodium glutamate in the medium were both over 18 g/L at the end of their additions,which basically reached their initial levels before the fermentation.At 56 h,almost all glutamate was depleted and the production of γ-GABA reached 22.5 g/L.Finally,the second addition of sodium glutamate was done as described above at 56 h.The production of γ-GABA was over 33 g/L at 104 h.关键词
γ-氨基丁酸/短乳杆菌/补料发酵Key words
γ-aminobutyric acid/Lactobacillus/batch fermentation分类
轻工纺织引用本文复制引用
谢晓阳,袁伟静,李昆太,郭晓燕,刘志文,徐波..高产γ-氨基丁酸富锌乳酸菌的分批及补料发酵研究[J].食品科学,2011,32(19):161-164,4.基金项目
国家自然科学基金项目 （）
江西省科技厅科技支撑项目 （）
江西农业大学博士科研启动基金项目 （）
