食品科学2011，Vol.32Issue(19)：174-179,6.
响应曲面法优化胰蛋白酶酶解条件制备乳源免疫活性肽
Optimization of Tryptic Hydrolysis Conditions for Preparing Milk-derived Immune Peptides by Response Surface Methodology
摘要
Abstract
Objective： To optimize the tryptic（PTN 6.0S） hydrolysis conditions of sodium caseinate for preparing peptides with high-immune activity.Methods： One-factor-at-a-time experimental design combined with response surface methodology was employed systematically to develop optimal hydrolysis conditions of sodium caseinate.The effect of hydrolyzed sodium caseinate on proliferation degree（stimulation index,SI） and ammonia nitrogen（AN） of mouse spleen cells was measured by tetrazolium salt-based colorimetric（MTT） assay.Results： The optimal hydrolysis conditions were substrate concentration of 3.5 g/100 mL,enzyme/substrate ratio of 3.8：1000,hydrolysis time of 15 h,hydrolysis temperature of 55.0 ℃ and hydrolysis pH of 7.0.Under the optimal hydrolysis conditions,the maximum SI was achieved to be 0.184±0.002 and the ammonia nitrogen content was up to（0.795±0.012） g/L.Conclusion： Enzymatic hydrolysis is a better way to prepare high-immune activity peptides from sodium caseinate.关键词
酪蛋白酸钠/免疫肽/MTT法/响应曲面法Key words
sodium caseinate/immune peptides/tetrazolium salt-based colorimetric（MTT） assay/response surface methodology分类
轻工纺织引用本文复制引用
徐鑫,何佳易,刘国艳,蔡丽丽,魏晓蕊,满其琪,张军..响应曲面法优化胰蛋白酶酶解条件制备乳源免疫活性肽[J].食品科学,2011,32(19):174-179,6.基金项目
江苏省高校自然科学基础研究项目 （）
扬州市-扬州大学合作基金项目 （）
