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响应曲面法优化植物乳杆菌胞外多糖的醇沉工艺

冯美琴 邱远 张琦 玛依诺·木图拉 陈晓红 董明盛

食品科学2011,Vol.32Issue(19):188-192,5.
食品科学2011,Vol.32Issue(19):188-192,5.

响应曲面法优化植物乳杆菌胞外多糖的醇沉工艺

Optimization of Ethanol Precipitation of Exopolysaccharides Produced by Lactobacillus plantarum 70810 by Response Surface Methodology

冯美琴 1邱远 2张琦 1玛依诺·木图拉 1陈晓红 1董明盛1

作者信息

  • 1. 南京农业大学食品科技学院,江苏南京210095
  • 2. 金陵科技学院动物科学与技术学院,江苏南京210038
  • 折叠

摘要

Abstract

The optimal conditions of ethanol precipitation for exopolysaccharides(EPS) produced by Lactobacillus plantarum 70810 were investigated by one-factor-at-a-time experiments and response surface methodology.A mathematical model was developed to reveal the significant effects of each factor and their interactions on the yield of EPS.The optimal parameters for ethanol precipitation of EPS from Lactobacillus plantarum 70810 were 12.56 h precipitation time,3.9:1 ethanol/fermentation supernatant ratio(V/V) and 3.54-fold condensation of fermentation supernatant.Under the optimal conditions,the model-predicted and experimental values of EPS yield were 323.11mg/L and 320.17 mg/L,respectively,with a relative error of 0.91%.

关键词

植物乳杆菌/胞外多糖/响应曲面法/醇沉

Key words

Lactobacillus plantarum/exopolysaccharides/response surface methodology/ethanol precipitation

分类

轻工纺织

引用本文复制引用

冯美琴,邱远,张琦,玛依诺·木图拉,陈晓红,董明盛..响应曲面法优化植物乳杆菌胞外多糖的醇沉工艺[J].食品科学,2011,32(19):188-192,5.

基金项目

国家“863”计划项目 ()

食品科学

OA北大核心CSCDCSTPCD

1002-6630

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