食品科学2011，Vol.32Issue(19)：227-231,5.
黄参多糖对癌细胞HepG2和P_（388）增殖抑制与细胞凋亡的影响
Effect of Sphallerocarpus gracilis Polysaccharides on Proliferation Inhibition and Apoptosis of Sarcoma HepG2 and Mouse Lymphocyte Leukemia P_（388）
摘要
Abstract
Objective： To explore the effect of Sphallerocarpus gracile polysaccharides（SGP） on apoptosis induction and proliferation inhibition of HepG2 and P388 cells as well as the lifespan extension of mice treated with SGP.Methods： HepG2 and P388 cells were co-cultured in the presence of SGP in this study.The inhibitory effect of SGP at different concentrations on the proliferation of HepG2 and P388 was evaluated by MTT assays.The SGP-induced apoptosis and cell cycle change of HepG2 and P388 were analyzed by flow cytometry.Meanwhile,the extension of lifespan of P388 mice treated with SGP was determined.Results： SGP revealed a significant anti-proliferation effect on HepG2 and P388 in vitro.The inhibition rate of SGP treatment for 48 h at the concentration of 200 mg/（kg·d） against HepG2 was 37.5%（P0.01）.The inhibition rates against P388 at 150 and 200 mg/（kg·d） after 48 h of treatment were both over 38%（P0.01）.In addition,SGP obviously inhibited the growth of implanted HepG2 in mice with an inhibitory rate of higher than 40%.Moreover,SGP induced the apoptosis of HepG2 and block HepG2 at the G1 phase.Furthermore,SGP prolonged the life of P388-bearing mice with a lifespan extension rate of 10%.Conclusion： SGP has an outstanding anti-cancer effect in mice and can effectively inhibit the proliferation of HepG2 and P388 cells and induce the apoptosis of HepG2 cells.关键词
黄参多糖/HepG2细胞/P388细胞/增殖抑制/细胞凋亡Key words
Sphallerocarpus gracilis polysacchorides（SGP）/HepG2 cell/P388/proliferation inhibition/apoptosis分类
医药卫生引用本文复制引用
贾磊,聂秀娟,方梅..黄参多糖对癌细胞HepG2和P_（388）增殖抑制与细胞凋亡的影响[J].食品科学,2011,32(19):227-231,5.基金项目
安徽省教育厅自然科学项目 （）
黄山学院引进人才启动项目 （）
