食品科学2011，Vol.32Issue(20)：121-125,5.
啤酒废酵母中β-D-葡聚糖非降解提取工艺
A Novel Method for Non-degradative Extraction of β-D-Glucans from Spent Yeast Cells
摘要
Abstract
We here present a novel method to extract non-degraded β-D-glucans from spent yeast cells,mainly based on induced yeast autolysis,hot water extraction,cell wall disruption,defatting and protease hydrolysis.One-factor-at-a-time coupled with orthogonal array design method was applied to the process conditions of induced yeast autolysis and cell wall disruption.The extract obtained under optimized conditions had a total sugar content of 84.9% with an extraction yield of 13.7%.Its purity and yield were both higher than previously reported.Induced yeast autolysis and hot water extraction had a significant effect on β-D-glucan purity and yield.Moreover,protease treatment could further remove protein impurities and increase β-D-glucan purity.The absence of strong acid,strong alkali and oxidant throughout the process helped to protect the physiological activity of products and the environment.关键词
废啤酒酵母/β-D-葡聚糖/非降解提取Key words
spent yeast cell/β-D-glucans/non-degradative extraction分类
轻工纺织引用本文复制引用
朱益波,翟丽君,朱明,齐斌..啤酒废酵母中β-D-葡聚糖非降解提取工艺[J].食品科学,2011,32(20):121-125,5.基金项目
江苏省高校自然科学研究计划项目 （）
