食品科学2011，Vol.32Issue(20)：160-163,4.
AccQ·Tag柱前衍生反相-高效液相色谱法测定齿果酸模中的氨基酸含量
Determination of Amino Acid Contents in the Whole Plant of Rumex dentatus by Reversed-Phase High-Performance Liquid Chromatography with AccQ·Tag Pre-column Derivatization Method
摘要
Abstract
Objective： To determine the composition and contents of amino acids in the whole plant of Rumex dentatus for further study of its nutritional characteristics and food value.Methods： Amino acids were derivatized by 6-aminoquinolyl-N-hydroxysuccinimidyl-carbamate（AQC）,and then their derivatives were separated on a Waters 515 HPLC system with a column temperature of 37 ℃ using phosphate buffer as mobile phase A and aqueous acetonitrile as mobile phase B in the gradient elution mode and detected at 248 nm using a UV detector.Results： Totally 17 amino acids were found,and the total content of amino acids in samples was 252.35 mg/g.The correlation coefficients of the calibration curves for 17 amino acids were all above 0.9995（n = 5）.The average spike recoveries of 17 amino acids were between 97.1% and 102.6% with a relative standard deviation（RSD） between 0.3% and 3.26%（n = 3）.关键词
齿果酸模/AccQ·Tag柱前衍生/反相-高效液相色谱法/氨基酸Key words
Rumex dentatus/AccQ·Tag pre-column derivatization/reversed-phase high-performance liquid chromatography（RP-HPLC）/amino acids分类
轻工纺织引用本文复制引用
高向阳,刘娜..AccQ·Tag柱前衍生反相-高效液相色谱法测定齿果酸模中的氨基酸含量[J].食品科学,2011,32(20):160-163,4.基金项目
河南省重点学科建设项目 （）
