食品科学2011，Vol.32Issue(21)：48-51,4.
两种石耳中多糖的提取与分离纯化及其单糖组成分析
Isolation,Purification and Monosaccharide Composition Analysis of Polysaccharides from Two Umbilicaria Species
摘要
Abstract
Objective：To analyze the monosaccharide composition of polysaccharides extracted from two Umbilicaria species： Umbilicaria decussate（Vill.） Zahlbr and Umbilicaria virginis Schaer..Methods： Water-soluble polysaccharides from both Umbilicaria species were obtained by water extraction,ethanol precipitation and freeze-dried and purified into fractions SUv and ZUd,respectively by sequential column chromatography on DEAE-52 and Sephadex G-150.Gas chromatography（GC） was used to analyze their monosaccharide composition.Results： The monosaccharide composition of crude Umbilicaria virginis polysaccharides was composed of mannose,glucose and galactose at a ratio of 16.00：50.96：6.17.The monosaccharide composition of SUv was comprised of mannose,glucose and galactose at a ratio of 2.97：17.70：1.52.The monosaccharide composition of crude Umbilicaria decussate polysaccharides was made up of mannose,glucose and galactose at a ratio of 3.90：38.086：2.00.ZUd contained only 5.37% glucose.Conclusion： Both Umbilicaria decussata and Umbilicaria virginis contain glucose and a small amount of mannose and galactose.关键词
网脊石耳/淡肤根石耳/气相色谱/单糖Key words
Umbilicaria decussate（Vill.） Zahlbr./Umbilicaria virginis Schaer./gas chromatography/polysaccharides/monosaccharides分类
轻工纺织引用本文复制引用
古丽扎尔·阿布都克依木,努尔比亚·亚力坤,克热木江·吐尔逊江,阿不都拉·阿巴斯..两种石耳中多糖的提取与分离纯化及其单糖组成分析[J].食品科学,2011,32(21):48-51,4.基金项目
国家自然科学基金项目（30960003 （）
31040004） （）
