食品科学2011，Vol.32Issue(21)：152-155,4.
分步酶解酪蛋白制备小分子ACE抑制肽
Preparation of Angiotensin-converting Enzyme Inhibitory Peptides with Low Molecular Mass from Casein by Stepwise Enzymatic Hydrolysis
摘要
Abstract
The aim of this study was to prepare ACE inhibitory peptides with low molecular mass from casein by stepwise hydrolysis with a single enzyme followed by double enzymes as a mimic for the gastrointestinal tract digestive system.Peptides with high ACE inhibitory activity were first produced by hydrolyzing casein under optimized operating conditions and then further hydrolyzed by trypsin and chymotrypsin together into small peptides still having high ACE inhibitory activity.The optimal conditions for pepsin hydrolysis were enzyme/substrate ratio of 6%,substrate concentration of 0.015 g/mL,initial hydrolysis pH of 1.8,hydrolysis temperature of 37 ℃ and hydrolysis time of 2 h,and the ACE inhibitory rate of the 10-fold diluted hydrolysate obtained was 84.5%.The optimal conditions for further hydrolysis by trypsin and chymotrypsin together were trypsin/chymotrypsin ratio of 2：1（m/m）,initial hydrolysis pH of 7.8,hydrolysis temperature of 48℃ and hydrolysis time of 5 h,and the resulting 10-fold diluted hydrolysate showed an ACE inhibitory rate of 85.9% and a molecular mass of less than 500 D.Thus,the stepwise enzymatic hydrolysis process proposed in this study can enable the preparation of casein-derived small peptides with high ACE inhibitory activity.关键词
酪蛋白/血管紧张素转化酶（ACE）/活性肽/分步水解Key words
casein/angiotensin-converting enzyme（ACE）/active peptide/stepwise hydrolysis分类
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王桂春,吕兵..分步酶解酪蛋白制备小分子ACE抑制肽[J].食品科学,2011,32(21):152-155,4.基金项目
江苏省自然科学基金项目 （）
