食品科学2011，Vol.32Issue(21)：183-187,5.
高产蛋白酶细菌的分离筛选及其种类鉴定
Screening and Identification of High Protease-producing Bacteria
马桂珍 1暴增海 1王淑芳 1吴少杰 1付泓润 1葛平华1
作者信息
- 1. 淮海工学院海洋学院,江苏连云港222005
- 折叠
摘要
Abstract
Totally 44 bacterial strains were isolated from 9 soil samples collected from Guangdong,Hebei,Liaoning,Hunan and Jiangsu by diluted plate method.The protease-producing ability of the bacteria isolated was screened by transparent cycle plate assay.The results indicated that of these bacterial isolates,16 exhibited clear transparent cycles.Meanwhile,strains G1-b,GD-2-2 and HX-B-1 revealed the strongest protease activity.The ratio of transparent circle diameter to colony diameter was higher than 3.5.Strain GD-2-2 had stronger protease activity than the other two,reaching 447.6 U/mL.Based on morphological observations and physiological and biochemical tests,strain GD-2-2 belonged to Bacillus.The 16S rDNA sequence analysis showed that the isolate was 100% similar with Bacillus licheniformis strain YB915（GQ996726.1）.Therefore,strain GD-2-2 belonged to Bacillus licheniformis.关键词
细菌/蛋白酶/鉴定Key words
bacteria/protease/identification分类
生物科学引用本文复制引用
马桂珍,暴增海,王淑芳,吴少杰,付泓润,葛平华..高产蛋白酶细菌的分离筛选及其种类鉴定[J].食品科学,2011,32(21):183-187,5.
