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混合反胶团萃取α-淀粉酶OA北大核心CSCDCSTPCD

Purification of α-Amylase Using CTAB/Tween-60 Mixed Reverse Micellar System

中文摘要英文摘要

以十六烷基三甲基溴化铵(CTAB)和脱水山梨醇单硬脂酸酯聚氧乙烯醚(Tween-60)为混合表面活性剂溶于正丁醇-异辛烷中构成反胶团系统,萃取纯化α-淀粉酶。研究不同萃取条件下,α-淀粉酶的萃取率。其中反胶团相组成为:ρ(CTAB+Tween-60)=4g/L;n(CTAB):n(Tween-60)=2.0:1.0;V(正丁醇):V(异辛烷)=1.0:1.0。水相组成为:α-淀粉酶配制的粗酶液,此时c(NaCl)=0.04mol/L,水相pH11…查看全部>>

Reverse micellar system consisting of the surfactants cetyltrimethyl ammonium bromide(CTAB) and Tween-60 in dissolved in n-butanol-isooctane mixture was used for α-amylase purification by reversed micellar extraction.Extraction efficiency of α-amylase was investigated under different extraction conditions using a reverse micellar phase consisting of 4 g/L(CTAB +Tween-60,2.0:1.0,n/n) in n-butanol-isooctane mixture(1.0:1.0,V/V) and an aqueous phase made up of …查看全部>>

高树刚;宋伟明;安红

淮海工学院化工学院,江苏连云港222005齐齐哈尔大学化学化工学院,黑龙江齐齐哈尔161006淮海工学院化工学院,江苏连云港222005

化学化工

十六烷基三甲基溴化铵(CTAB)吐温-60反胶团α-淀粉酶萃取

cetyltrimethylammonium bromide(CTAB)Tween-60reverse micelleα-amylaseextraction

《食品科学》 2011 (21)

214-217,4

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