食品科学2011，Vol.32Issue(22)：20-24,5.
花果山墨旱莲多糖的提取及体外抗氧化活性比较
Extraction of Polysaccharides from Eclipata Alba Grown in Huaguo Mountain and Comparison of Antioxidant Activity in vitro with Total Flavonoids from Eclipata Alba
摘要
Abstract
Using one-factor-at-a-time experiments followed by orthogonal array design,the process conditions of polysaccharide extraction from Eclipata Alba grown in Huaguo Mountain were optimized in this study.Also,a comparison of antioxidant activity was carried out between polysaccharides（PEA） and flavonoids（FEA） from Eclipata Alba.The results showed that the optimum conditions for polysaccharide extraction were material-to-liquid ratio of 1：15（g/mL）,extraction time of 1 h,extraction temperature of 90 ℃ and extraction number of 3.After the removal of impurities such as proteins,nucleic acids,etc,the extracted polysaccharides were characterized using ultraviolet spectroscopy and infrared spectroscopy.Both PEA and FEA had a powerful ability to scavenge hydroxyl and superoxide anion radicals and could scavenge the former more powerfully.At the same concentrations exceeding 0.085 mg/mL,FEA indicated stronger antioxidant activity than PEA.关键词
花果山墨旱莲/多糖/黄酮类化合物/提取/抗氧化活性Key words
Eclipata Alba grown in Huaguo Mountain/polysaccharides/flavonoids/extraction/antioxidant activity分类
医药卫生引用本文复制引用
许瑞波,王新新,唐秋萍,胡喜兰,詹永成..花果山墨旱莲多糖的提取及体外抗氧化活性比较[J].食品科学,2011,32(22):20-24,5.基金项目
江苏省海洋生物技术重点建设实验室开放课题 （）
江苏省青蓝工程优秀青年骨干教师资助项目 （）
淮海工学院博士科研启动项目 （）
