食品科学2011，Vol.32Issue(22)：114-118,5.
响应面法优化金乌贼眼透明质酸的提取工艺
Optimization of Extraction Process for Hyaluronic Acid from Golden Cuttlefish Eyes Using Response Surface Methodology
摘要
Abstract
In order to make full use of golden cuttlefish eyes,the enzymatic preparation of hyaluronic acid（HA） from the waste of golden cuttlefish processing was studied.The results of a comparison of 3 proteases showed that neutral protease was more suitable to hydrolyze golden cuttlefish eyes than pepsin and trypsin,providing an HA extraction yield of 6.64%.Employing one-factor-at-a-time experiments and response surface methodology,the optimal conditions for HA extraction by neutral protease-catalyzed hydrolysis were determined as follows： 8200 U/g enzyme amount and 60 ℃ for a reaction duration of 3 h.Under these conditions,the experimental extraction yield of HA was up to 10.70%,in close agreement with the predicted value.Accordingly,the established extraction process was accurate and feasible.It was also found that enzyme amount was the most important fact the affects extraction yield of HA followed by temperature and reaction time.关键词
响应面/金乌贼眼/透明质酸/提取Key words
response surface methodology/golden cuttlefish eyes/hyaluronic acid/extraction分类
医药卫生引用本文复制引用
张丽媛,李和生,孙楠楠,雷静..响应面法优化金乌贼眼透明质酸的提取工艺[J].食品科学,2011,32(22):114-118,5.基金项目
浙江省科技厅重大攻关项目 （）
