食品科学2011，Vol.32Issue(22)：226-229,4.
细脚拟青霉甘露醇含量的测定
Determination of Mannitol in Paecilomyces tenuipes Mycelium and Its Culture Medium
刘桂君 1尚宏忠 2李赛飞 1文华安2
作者信息
- 1. 北京市辐射中心微生物辐射育种实验室,北京100012
- 2. 中国科学院微生物研究所,北京100101
- 折叠
摘要
Abstract
The mannitol content of the mycelium of Paecilomyces tenuipes and its culture medium was analyzed colorimetrically at 412 nm.The effects of extraction solvent,temperature and time on extraction yield of mannitol were investigated to optimize the extraction conditions.The best results of mannitol extraction were achieved by using distilled water to extract Paecilomyces tenuipes mycelium at 60 ℃ and extract its culture medium at 100 ℃ for 60 min both.Under these conditions,the recovery rate of mannitol from Paecilomyces tenuipes mycelium was 99.94% with a RSD of 0.74%,the precision RSD was 0.80% and a good stability of the OD value at 412 nm was found within 60 min.In the case of mannitol extraction from the culture medium,the recovery,RSD and precision RSD were 99.79%,1.17%,and 1.00%,respectively,and the OD value at 412 nm also showed a good stability within 60 min.关键词
细脚拟青霉/甘露醇/比色法/菌丝体/固体培养物Key words
Paecilomyces tenuipes/mannitol/colorimetry/mycelium/medium分类
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刘桂君,尚宏忠,李赛飞,文华安..细脚拟青霉甘露醇含量的测定[J].食品科学,2011,32(22):226-229,4.
