食品科学2011，Vol.32Issue(22)：280-284,5.
HPLC-MS/MS测定玉米中异恶唑草酮及代谢物残留量
Determination of Isoxaflutole and Its Metabolite Residues in Maize by HPLC-MS/MS
摘要
Abstract
An HPLC-MS/MS（high performance liquid chromatography coupled with tandem mass spectrometry） method has been developed for the determination of isoxaflutole and its two metabolite residues in maize.The isoxaflutole and its metabolite residues in samples were extracted into acetic acid-cetonitrile（1：99,V/V） by means of high-speed homogenization,subjected to liquid-liquid partition in the presence of added anhydrous magnesium sulfate and sodium acetate,cleaned up with primary secondary amine（PSA）,octadecylsilane（ODS） and graphitized carbon black for purpose of eliminating such most matrix interferences as lipids,pigments,etc before HPLC-MS/MS analysis and subsequent quantification by an external standard method.The average recovery rates of isoxaflutole and its metabolites from spiked samples ranged between 74.8% and 107.2% with a relative standard deviation of 6.45%-11.15% across the spike levels of 0.010,0.050 mg/kg and 0.100 mg/kg.The detection limit was 0.01 mg/kg and 0.005 mg/kg for isoxaflutole and its metabolites,respectively.This method was sensitive,accurate,time-saving,simple,and suitable for the detection of isoxaflutole and its metabolite residues in maize.关键词
异恶唑草酮及代谢物/高效液相色谱-串联质谱（HPLC-MS/MS）/玉米Key words
isoxaflutole and its metabolites/high performance liquid chromatography coupled with tandem mass spectrometry（HPLC-MS/MS）/maize分类
轻工纺织引用本文复制引用
杨长志,王传松,程阳,刘永,吴岩,魏冬旭..HPLC-MS/MS测定玉米中异恶唑草酮及代谢物残留量[J].食品科学,2011,32(22):280-284,5.基金项目
国家认证认可管理委员会检验检疫行业标准制订项目 （）
