食品科学2011，Vol.32Issue(23)：58-63,6.
白背三七多糖的分离纯化
Separation and Purification of Polysaccharides from Leaves of Gynura davaricata（L.） DC.
刘微微 1刘旭 1曹学丽1
作者信息
- 1. 北京工商大学食品学院,食品添加剂与配料北京高校工程研究中心,北京100048
- 折叠
摘要
Abstract
Two neutral polysaccharides（GDPs-1 and GDPs-2） and one acidic polysaccharide（GDPs-3） were separated and purified from water-soluble crude polysaccharide from the leaves of Gynura davaricata（L.） DC.by D315 resin column and subsequent Sephadex G-25 gel column chromatography.GPC analysis showed that the average molecular weights of GDPs-1,GDPs-2 and GDPs-3 were 1.06 × 104,4.17 × 103 D,and 3.74 × 103 D with a purity of 94.08%,92.3% and 99.8%,respectively.Infrared spectra preliminarily confirmed that the purified fractions were polysaccharides.GDPs-1 was characterized as α-and β-configuration,and GDPs-2 and GDPs-3 asα-configuration.Nuclear magnetic resonance（1H-NMR） results further proved that the polysaccharides contained only α-configuration.In conclusion,all these investigations can provide reference data for further studies on structural characterization and bioactivity of Gynura davaricata polysaccharides.关键词
白背三七/多糖/分离纯化/红外光谱/构型/核磁共振Key words
Gynura davaricata（L.） DC./polysaccharide/separation and purification/infrared spectrum/configuration/nuclear magnetic resonance分类
化学化工引用本文复制引用
刘微微,刘旭,曹学丽..白背三七多糖的分离纯化[J].食品科学,2011,32(23):58-63,6.
