食品科学2011，Vol.32Issue(23)：260-265,6.
嗜酸乳杆菌细胞壁蛋白酶的提取优化
Optimization of Extraction of Cell-envelope Proteinase from Lactobacillus acidophilus
摘要
Abstract
In this study,the extraction of cell-envelope proteinase（CEP） from Lactobacillus acidophilus was optimized.A comparison of ultrasonic treatment,Ca2＋-free treatment and lysozyme lysis for their effectiveness in Lactobacillus acidophilus cell disruption was performed to determine lysozyme lysis as the best method among the three.Further,process conditions optimization for CEP extraction from Lactobacillus acidophilus by lysozyme lysis was carried out using orthogonal array design based on one-factor-at-a-time experiments.The best extraction results were obtained by 3 h incubation at 37 ℃ of Lactobacillus acidophilus cells in pH 7.8 lysozyme lysis buffer containing 50 mmol/L Tris-HCl,100 mmol/L NaCl,1% TritonX-100 and 1.5 mg/mL lysozyme and then centrifugation at 4500 r/min and 4 ℃ for 20 min,and the resulting supernatant was collected as CEP.In addition,lysozyme concentration had the largest impact on extraction efficiency of CEP followed by pH,Tirton X-100 concentration and incubation time.关键词
嗜酸乳杆菌/细胞壁蛋白酶（CEP）/提取Key words
Lactobacillus acidophilus/cell-envelope proteinase（CEP）/extraction分类
轻工纺织引用本文复制引用
李敏,潘道东..嗜酸乳杆菌细胞壁蛋白酶的提取优化[J].食品科学,2011,32(23):260-265,6.基金项目
国家自然科学基金项目 （）
江苏省“十一五”科技支撑计划项目 （）
国家农业科技成果转化基金项目 （）
