食品科学2011，Vol.32Issue(23)：270-276,7.
复合诱变选育表面活性素（surfactin）高产菌株
Breeding of High-yield Surfactin-producing Strain by a Combined Mutagenesis Approach
摘要
Abstract
In order to breed a high-yield surfactin-producing strain,Bacillus subtilis fmbJ224 was used as the start strain and mutagenized sequentially with lithium chloride（LiCl）,nitrosoguanidine（NTG）,ethyl methane sulphonate（EMS） and streptomycin.The preliminary and secondary screening results showed that strain LN2-3 had high ability to produce surfactin,with a yield of 239.2 mg/L,which revealed a 44.56-fold increase compared with the original strain.Meanwhile,the strain had good genetic stability.This study demonstrates that the mutation rate of the original strain could be raised greatly by the combined use of LiCl and NTG.关键词
芽孢杆菌/亚硝基胍（NTG）/LiCl/复合诱变/表面活性素Key words
Bacillus subtilis/nitrosoguanidine（NTG）/LiCl/combined mutagenesis/surfactin分类
轻工纺织引用本文复制引用
郭芳芳,李金良,陆兆新,别小妹,张充,吕凤霞..复合诱变选育表面活性素（surfactin）高产菌株[J].食品科学,2011,32(23):270-276,7.基金项目
“十二五”国家科技支撑计划项目 （）
国家自然科学基金项目 （）
