食品科学2011，Vol.32Issue(24)：1-6,6.
利用大孔吸附树脂吸附分离藏红花细胞培养液中藏红花素和藏红花苦素
Separation of Crocin and Picrocrocin from Saffron Cell Culture Broth by Macroporous Resin Adsorption
摘要
Abstract
Objective： The separation of crocin and picrocrocin from saffron cell culture broth was investigated by macroporous resin adsorption.Methods： The effectiveness of 4 types of macroporous resin was compared in separating crocin and picrocrocin.The application of HPD-100A resin for separating crocin and picrocrocin from saffron cell culture broth was optimized.Results： HPD-100A resin was the best resin to separate crocin and picrocrocin from saffron cell culture broth among the investigated ones.The best separation of crocin and picrocrocin was achieved as an adsorption rate of 94.4% and 75.5% and a desorption rate of 99.9% and 87.5%,respectively when the cell culture broth was concentrated until a concentration of 1.0 mg/mL and 24.6 mg/mL for crocin and picrocrocin,respectively,temperature 25 ℃,pH 6,and 1.5 BV of each concentrate was loaded onto the column at a flow rate of 1.5 mL/min after being adjusted to pH 6 and then eluted with 1.7 BV of 40% aqueous ethanol at a flow rate of 1.0 mL/min.Conclusion： Macroporous resin adsorption is feasible and holds promise for separating crocin and picrocrocin from saffron cell culture broth.关键词
大孔吸附树脂/藏红花素/藏红花苦素/提取/藏红花细胞培养Key words
macroporous adsorption resin/crocin/picrocrocin/extraction/saffron cell culture分类
轻工纺织引用本文复制引用
吴频梅,袁丽红,黄晶,孙镇..利用大孔吸附树脂吸附分离藏红花细胞培养液中藏红花素和藏红花苦素[J].食品科学,2011,32(24):1-6,6.基金项目
国家“973”项目 （）
