食品科学2011，Vol.32Issue(24)：64-68,5.
功能性骨胶原多肽提取前处理的脱脂脱钙条件研究
Optimization of Process Condition for Defatting and Decalcification of Bovine Bone before Extraction of Functional Collagen Polypeptides
摘要
Abstract
The defatting and decalcification of bovine bone paste before extraction of functional collagen ploypeptides was optimized with respect to the selection of defatting agent and decalcification conditions including citric acid concentration,soaking time and solid-to-liquid ratio.Absolute ethanol was found to be the best defatting solvent among the investigated three.Following one-factor-at-a-time experiments,a quadratic orthogonal rotation combination design was performed to optimize the decalcification conditions.The best results for the defatting and decalcification of bovine bone paste were obtained as a defatting rate of 97.46% and a decalcification rate of 96.53% by suspension in absolute ethanol at a solid-to-liquid ratio of 1：20（g/mL） with shaking at 150 r/min and then decalcification for 16 h with 0.43 mol/L citric acid at a solid-to-liquid ratio of 1：21（g/mL）.关键词
骨泥/脱脂/脱钙/二次正交旋转组合设计Key words
bone paste/defatting/decalcification/quadratic orthogonal rotation combination design分类
轻工纺织引用本文复制引用
唐莹莹,罗爱平,何光中,郎小琴,陈维..功能性骨胶原多肽提取前处理的脱脂脱钙条件研究[J].食品科学,2011,32(24):64-68,5.基金项目
贵州省科技厅资助项目 （）
贵州省肉牛产业技术体系建设专项 （）
