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酶法提取白酒糟中酚酸物质工艺

王鑫 刘微微 曹学丽

食品科学2011,Vol.32Issue(24):114-119,6.
食品科学2011,Vol.32Issue(24):114-119,6.

酶法提取白酒糟中酚酸物质工艺

Enzymatic Extraction of Phenolic Components from Distiller's Grains

王鑫 1刘微微 2曹学丽1

作者信息

  • 1. 北京工商大学食品学院
  • 2. 食品添加剂与配料北京高校工程研究中心,北京100048
  • 折叠

摘要

Abstract

An enzymatic hydrolysis method was proposed to extract phenolic components from distiller's grains.The extraction process was optimized by quadratic rotation-orthogonal composite experimental design method.The extraction yield of total phenolic acids was investigated with respect to variable parameters including enzyme dosage(X1),reaction time(X2),pH(X3) and material-to-liquid ratio(X4).A regression model was established as Y=1.28321+0.04783X1+0.03728X2+0.12048X3+0.05152X4-0.05027X12+0.01514X22+0.00099X32-0.03790X42+0.02000X1X2+0.03000X1X3+0.00250X1X4.The optimal extraction conditions were enzyme dosage 10 mL/100 g,pH 6.6,and material-to-liquid ratio 1:25,and hydrolysis time 150 min.Under these conditions,the predicted and actual yields of total phenolic acids were 1.50% and 1.51%,showing a relative error of 0.67%.HPLC analysis indicated that an obvious increase of tran-ferulic acid after enzymatic hydrolysis was observed.

关键词

白酒糟/酚酸/酶解/提取/工艺优化

Key words

distiller/s grains/phenolic acids/enzymatic hydrolysis/extraction/optimization

分类

轻工纺织

引用本文复制引用

王鑫,刘微微,曹学丽..酶法提取白酒糟中酚酸物质工艺[J].食品科学,2011,32(24):114-119,6.

食品科学

OA北大核心CSCDCSTPCD

1002-6630

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