食品科学2012，Vol.33Issue(1)：20-24,5.
广林9号桉叶多酚抗氧化活性研究
Antioxidant Activity of Polyphenol Extracts from Leaves of E.grandis×E.urophylla Guanglin No.9
摘要
Abstract
Crude ethanol extract from the leaves of E.grandis×E.urophylla Guanglin No.9 was fractionated by repeated extractions sequentially with solvents of different polarity into petroleum ether-soluble,chloroform-soluble,ethyl acetate-soluble,n-butanol-soluble fractions and residue.Total phenolic contents in the six samples were determined by Folin-Ciocalteu method.Meanwhile,their antioxidant activity was tested by in vitro ABTS,DPPH and FRAP assays.The results indicated that among the samples,the ethyl acetate-soluble fraction showed the highest total phenolic content,502.67 mg/g,and the strongest ABTS and DPPH scavenging activity and reducing power activity,which were better than those of VC.The IC50 values of the fraction against DPPH and ABTS radicals were 0.097 mg/mL and 0.034 mg/mL,respectively.Moreover,a positive correlation between total phenolic content and antioxidant activity was observed for all the samples.This indicates that polypehnolics are mainly responsible for their antioxidant activity.The crude ethanol extract exhibited a total phenloic content（over 30%） similar to that of its counterpart from tea.This study demonstrates that the crude ethanol extract has a high exploitation potential.关键词
桉叶/多酚/抗氧化能力/1,1-二苯基-2-三硝基苯肼（DPPH）/2,2＇-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）Key words
eucalyptus leaf/polyphenol/antioxidant activity/DPPH/ABTS分类
农业科技引用本文复制引用
王俊亮,肖苏尧,陈运娇,陈雪香,汤杰,曹庸..广林9号桉叶多酚抗氧化活性研究[J].食品科学,2012,33(1):20-24,5.基金项目
国家林业公益性行业科研专项 （）
