食品科学2012，Vol.33Issue(2)：53-57,5.
碱性蛋白酶酶解罗非鱼下脚料蛋白动力学研究
Kinetic Model for Enzymatic Hydrolysis of Protein from Tilapia Waste
摘要
Abstract
Tilapia waste was used as the material to conduct hydrolysis by alkaline protease.The degree of hydrolysis（DH） of protein from tilapia waste was determined to explore the optimal enzymatic hydrolysis conditions.The results indicated that the optimal hydrolysis conditions for tilapia waste were hydrolysis temperature of 50 ℃,substrate concentration of 20 g/L,enzyme concentration of 0.067 g/L,hydrolysis pH of 9.50 and hydrolysis time of 180 min.Under the optimal hydrolysis conditions,the DH of tilapia waste was 22%.Meanwhile,a kinetic model of hydrolysis was established,which revealed an inactivation constant（Kd） of 37.9 min-1for enzyme,enzymatic reaction rate constant（K2） of 244.2 min-1,Michaelis-Menten constant（KM） of 29.27 g/L,and the maximum reaction rate（Vmax） of 28.41 g/（min.L） at 50 ℃.The kinetic equation of enzymatic hydrolysis of tilapia waste protein was derived.The proposed kinetic model exhibited a convenient determination of kinetic parameters by a few experiments.关键词
罗非鱼下脚料/碱性蛋白酶/酶解/动力学Key words
tilapia waste/alkaline protease/enzymatic hydrolysis/kinetics分类
轻工纺织引用本文复制引用
黄秋霞,孙建华,兰雄雕,蒋林斌,童张法,廖丹葵..碱性蛋白酶酶解罗非鱼下脚料蛋白动力学研究[J].食品科学,2012,33(2):53-57,5.基金项目
广西科学研究与技术开发项目（桂科攻0992025-17 （）
桂科攻0996033 （）
桂科攻10123008-20） （）
