食品科学2012，Vol.33Issue(2)：216-218,3.
高效液相色谱法测定牛奶中的胆固醇
Determination of Cholesterol in Milk by HPLC
张丽 1汤佳佳 1徐国兵 2耿超2
作者信息
- 1. 安徽中医学院药学院,安徽合肥230031
- 2. 安徽省食品药品检验所,安徽合肥230051
- 折叠
摘要
Abstract
Objective： To establish an HPLC method for the determination of cholesterol in milk.Methods： Cholesterol was separated on a Shimadzu C18column（4.6 mm × 250 mm,5μm） using acetonitrile as mobile phase at a flow rate of 1.0 mL/min.The detection wavelength was set at 203 nm and the column temperature at 30 ℃.Results： A calibration curve was established revealing a good linear relationship in the range of 3.58-358μg/mL with a correlation coefficient of 0.9999.The average spike recovery rate was 101.8% with a RSD of 1.14%.Conclusion： The method was accurate,reproducible and applicable for the determination of cholesterol in milk.关键词
高效液相色谱法/牛奶/胆固醇Key words
high performance liquid chromatography（HPLC）/milk/cholesterol分类
化学化工引用本文复制引用
张丽,汤佳佳,徐国兵,耿超..高效液相色谱法测定牛奶中的胆固醇[J].食品科学,2012,33(2):216-218,3.
