食品科学2012，Vol.33Issue(2)：231-234,4.
固相萃取-高效液相色谱-串联质谱法测定食品中的丙烯酰胺
Determination of Acrylamide in Foods by Solid-phase Extraction and HPLC-MS/MS
摘要
Abstract
A method using [D3]-acrylamide as an isotope internal standard for determining acrylamide in foods was established by solid-phase extraction coupled with high performance liquid chromatography and quadrupole tandem mass spectrometry（HPLC-MS/MS）.Analytical procedures were composed of amylase hydrolysis at a concentration of 20 mg/mL,the removal of fat,cleanup by solid-phase extraction（SPE） and the determination by HPLC-MS/MS with a multi-reaction monitoring（MRM） mode and the addition of an internal standard.The limit of detection（LOD） and limit of quantitation（LOQ） of the developed method were 4.8 and 16.5μg/kg,respectively.This method has the advantages of convenient operation,high sensitivity,excellent accuracy and good reproducibility and is therefore suitable for the determination of acrylamide in foods.关键词
高效液相色谱-串联质谱/固相萃取/丙烯酰胺/同位素内标Key words
high performance liquid chromatography and quadrupole tandem mass spectrometry（HPLC-MS/MS）/solid phase extraction/acrylamide/isotope internal standard分类
轻工纺织引用本文复制引用
程雷,郑炎夏,徐虹,孙宝国..固相萃取-高效液相色谱-串联质谱法测定食品中的丙烯酰胺[J].食品科学,2012,33(2):231-234,4.基金项目
北京市留学人员科技活动择优资助项目 （）
北京市属高等学校科学技术与研究生创新工程建设项目 （）
北京工商大学2011年本科生科学研究计划项目 （）
