食品与发酵工业2011，Vol.37Issue(6)：1-5,5.
麦芽糖α-淀粉酶基因在枯草芽孢杆菌中的分泌表达
Secreted Expression of the Maltogenic Amylase in Bacillus subtilis
摘要
Abstract
The gene encoding maltogenic amylase was fused with signal peptide gene encoding amylase of Bacillus licheniformis by overlapping PCR.The fused gene（BlMa） was ligated with an expression vector of Bacillus,pHY-P43.B1Ma transforms Bacillus subtilis directly and results in the recombinant plasmid pHY-P43-BlMa.Bacillus subtilis 1A717 which is deficient in amylase was selected as expression host.The recombinant strain was designated as Bacillus subtilis / pHY-P43-BlMa.Enzyme activity assay and SDS-PAGE showed that recombinant maltogenic amylase of B.licheniformis（BlMa enzyme）expressed by B.subtilis / pHY-P43-BlMa was secreted into medium.High Performance Liquid Chromatography（HPLC） identified that major hydrolysate of starch catalyzed by BlMa enzyme was maltose.Culture condition of Bacillus subtilis / pHY-P43-BlMa in flask scale was optimized for production of BlMa enzyme,the optimized medium was： 10 % corn starch,2.5 % cotton seed protein,0.3 %（NH4）2SO4,0.03 % CaCl2 and 0.1 % NaH2PO4.The maximum enzyme activity under optimized condition reached 5.9 U/mL.关键词
麦芽糖α-淀粉酶/分泌表达/枯草芽孢杆菌Key words
maltogenic amylase/secreted expression/Bacillus subtilis分类
生物科学引用本文复制引用
沈微,俞玲,陈献忠,樊游,王正祥..麦芽糖α-淀粉酶基因在枯草芽孢杆菌中的分泌表达[J].食品与发酵工业,2011,37(6):1-5,5.基金项目
国家863高技术研究发展计划 （）
