食品与发酵工业2011，Vol.37Issue(6)：161-164,4.
转基因玉米的七重PCR鉴定方法
Seven-Plex PCR Detection Method for Genetically Modified Maize
摘要
Abstract
To establish Seven-plex PCR detection system for detecting transgenic components in genetically modified Maize,endogenous gene IVR,exogenous herbicide gene BAR and PAT,exogenous insect resistant gene Cry1Ab,screening gene NPTII,CaMV35S promoter and Nos terminator were chosen for the targeted fragment.7 pairs of primers are designed and the PCR conditions for the multiplex PCR analysis were optimized according to the primer concentration and annealing temperature.The results show that Seven-Plex PCR system can detect endogenous gene and transgenic components with high efficiency and good stability.关键词
玉米/转基因成分/七重PCR检测Key words
maize/genetically modified components/Seven-Plex PCR detection分类
农业科技引用本文复制引用
陈贞,芦春斌,杨梦婕,马骏,白卫斌,吴希阳..转基因玉米的七重PCR鉴定方法[J].食品与发酵工业,2011,37(6):161-164,4.基金项目
农业部转基因重大专项“转基因生物检测和监测新技术” （）
