食品与发酵工业2011，Vol.37Issue(7)：28-33,6.
产耐热纤维素酶菌株的分子鉴定及产酶条件优化
Molecular Identification and Characterization of a Thermostable Cellulase-Secreting Strain
陈红漫 1孙玉辉 1阚国仕 1任大明 1杨佳颖 1蔡金涛1
作者信息
- 1. 沈阳农业大学生物科学技术学院,辽宁沈阳110866
- 折叠
摘要
Abstract
A thermophilic strain NR615 possessing cellulase was isolated at 60℃ from hot spring samples. Based on the ITS sequence and phylogenetic tree analysis, strain NR615 was identified as Aspergillusfumigatus. The strain was capable of growing well at 28 - 60 ℃. Aeeording to optimization condition of cell growth and enzyme production, the initial pH is 6. 5. The supernatant of strain incubated medium showed efficient activity of cellulase with addition of crystalline cellulose as carbon and beef extract as nitrogen sources respectively. The highest enzyme activity at 2.41 IU/mL was reached after incubation for 5d. The optimal pH of the celullase for the reaction is 5.0. This enzyme shows stable aetivity over a broad range of temperature from 50 ℃ to 70 ℃. These characteristics of the eellulase demonstrated that it has potential application in the thermostable cellulase field.关键词
烟曲霉/系统发育进化/ITS序列分析/响应面分析Key words
Aspergillus fumigatus/phylogenetic analysis/ITS sequence analysis/response surface analysis分类
化学化工引用本文复制引用
陈红漫,孙玉辉,阚国仕,任大明,杨佳颖,蔡金涛..产耐热纤维素酶菌株的分子鉴定及产酶条件优化[J].食品与发酵工业,2011,37(7):28-33,6.
