食品与发酵工业2011，Vol.37Issue(7)：34-38,5.
玉米胚谷氨酸脱羧酶的性质
Some Properties of Glutamate Decarboxylase from Corn Germ
李楠 1王玲玲 1吴子健 1王文蒙1
作者信息
- 1. 天津市食品生物技术重点实验室,天津商业大学生物技术与食品科学学院,天津300134
- 折叠
摘要
Abstract
The properties of glutamate decarboxylase （GAD） from corn germ were investigated. The GAD showed its maximal activity at 40℃ and pHi. 7 respectively. The GAD was sensitive to temperature. Its activity remains 39% under 60 ℃ after lh, and it is inactive at 85~C. However, the GAD had a relatively wide pit range tor stability, whose activity remained at 80% under pH 4.5 -7.5. The Km and V of GAD for Glu were 51.87mnlOl/I. andl. 807mg/min respectively. Moreover, KCl, NaCl and SDS inhibited the GAD activity remarkably, whereas Ca^2＋ could activate the GAD, this showed that Ca^2＋ enhanced the affinity between enzyme and the substrate.关键词
谷氨酸脱羧酶/γ-氨基丁酸/玉米胚/酶学性质Key words
glutamate decarboxylase/γ-aminobutyric acid/corn germ/enzymatic properties分类
轻工纺织引用本文复制引用
李楠,王玲玲,吴子健,王文蒙..玉米胚谷氨酸脱羧酶的性质[J].食品与发酵工业,2011,37(7):34-38,5.
