食品与发酵工业2011，Vol.37Issue(7)：81-85,5.
阿卡波糖发酵过程的放大研究
Study on the Scaling-up of Acarbose Fermentation Processes
摘要
Abstract
The scale-up strategy for acarbose fermentation by Actinoplanes sp. A-56 was explored in this paper. The results obtained in shake-flask cultivation demonstrated that the ratio of maltose and glucose had significant effect on acarbose biosynthesis, and the feeding medium containing 3 ： 1 （ mass ratio） of maltose and glucose was favorable for acarbose production. Then the correlation of carbon source concentration with acarbose production was further in- vestigated in 100 L fermenter, and the results showed that 75 - 80 g/L of total sugar and 40 -45 g/L of reducing sugar were the optimal for acarbose production. Based on the results in 100 L fermenter, an effective and simplified scale-up strategy was successfully established for acarbose fermentation in a 30 m^3 fermenter by using total sugar and reducing sugar as the scale-up parameter. As a result, 4 327 mg/L of acarbose was obtained at 168h of fermentation.关键词
游动放线菌A-56/阿卡波糖/碳源/放大Key words
Actinoplanes sp. A-56/acarbose production/carbon source/scale-up分类
轻工纺织引用本文复制引用
叶亚健,庞会忠,周佳,程新,李昆太..阿卡波糖发酵过程的放大研究[J].食品与发酵工业,2011,37(7):81-85,5.基金项目
江西省科技支撑计划重点项目（2009BGA02100）和江西农业大学博士科研启动基金资助项目 （）
