食品与发酵工业2011，Vol.37Issue(8)：61-65,5.
透析培养法稳定出芽短梗霉摇瓶发酵条件的研究
Stabilize the Cultural Conditions of Aureobasidium pullulans by Dialysis Cultivation
摘要
Abstract
In this paper, the cell morphologies of Aureobasidium pulhdans G58-2 under the dialysis cultivation, and the optimal cultivation conditions for pullulan production were studied. The results showed that inoculation by dialysis cultivation was better than normal cultivation, OD600 of bacterial suspension was 0. 442, the inoculation time 36 h, initial pH7.0, 250 mL shake flask containing 50 mL of sucrose medium with 200 r/min. Under these conditions, the highest pullulan yield （4.84 g/100 mL） with the lowest pigment was obtained, and the stability of experiments effectively was improved.关键词
透析培养/菌悬液/出芽短梗霉/短梗霉多糖Key words
dialysis cultivation/bacterial suspension/Aureobasidium pullulans/pullulan分类
化学化工引用本文复制引用
刘畅,童群义..透析培养法稳定出芽短梗霉摇瓶发酵条件的研究[J].食品与发酵工业,2011,37(8):61-65,5.
