食品与发酵工业2011，Vol.37Issue(8)：122-125,4.
激光复合诱变选育那西肽高产菌及其代谢特性的研究
Breeding of High-yield Nosiheptide Strain by Laser Compound Mutation and Studying on Metabolic Properties
薛正莲 1秦艳飞 1王洲 1赵世光 1杨超英1
作者信息
- 1. 安徽工程大学生物与化学工程学院,微生物发酵安徽省工程技术研究中心,安徽芜湖241000
- 折叠
摘要
Abstract
The starting strain was treated by ultraviolet irradiation, then it was screened further by laser compound mutation and studied on the genetic stability to obtain the stable high-yield nosiheptide-producing strains. The result showed that the potency of strain NXT-U19 was increased by 44.22% compared to the original strain （546.17 μg/mL）, reaching 787.71 μg/mL. Six high-yield strains was screened by laser-ultraviolet compound mutation, among them the highest potency of strain NXT-U19-J2 was increased by 65.74% compared to the original strain, reaching 905.22 μg/mL. The analysis of subculture proved that the genetic stability of mutated strain NXT-U19-J2 was good. This result has showed that laser compound mutation is the efficient method to obtain the high-yield nosiheptideproducing strains.关键词
紫外/激光/复合诱变/那西肽Key words
ultraviolet/laser/compound mutation/nosiheptide分类
化学化工引用本文复制引用
薛正莲,秦艳飞,王洲,赵世光,杨超英..激光复合诱变选育那西肽高产菌及其代谢特性的研究[J].食品与发酵工业,2011,37(8):122-125,4.
