食品与发酵工业2011，Vol.37Issue(9)：208-211,4.
气相色谱法同时测定多糖中的中性糖、糖醛酸、氨基糖和唾液酸
Simultaneous Determination of Neutral Sugar, Uronic Acid, Amino Sugar and Sialic Acid of Polysaccharides by Gas Chromatography
摘要
Abstract
Neutral sugar, uronic acid, amino sugar and sialic acid were effectively determined simultaneously by gas chromatography after methanolysis and trimethylsilylation. Three standard monosaccharide mixtures （including Ara, Rha, Fuc, Xyl, Man, Gal, Gle, GlcA, GalA, GalNAc, GlcNAc, Neu5Ac） and two polysaccharide samples （okara polysaccharide DFP, Coprinus comatus polysaccharide F32） were methanolysis by 1 mol/L HC1/methanol at 85 ℃ for 18 -24 h, neutralized with Ag2CO3 and re-N-acetylated by adding acetic anhydride for 24 h in the dark. After removing the silver salts, the dried residue was trimethylsilylated by adding silylation reagent （pyridine/hexamethyldisilazane/chlorotrimethylsilane = 5： 1：1 ） for 30 min at room temperature. Then the final products were analyzed by gas chromatography. This method needs fewer amount of sample, and has higher sensitivity and resolution. It can simultaneous detect kinds of monosaccharides. It will have a great potential for application.关键词
气相色谱/单糖组成/糖醛酸/氨基糖/多糖Key words
gas chromatography/monosaccharide composition/uronic acid/amino sugar/polysaccharide分类
化学化工引用本文复制引用
李波,芦菲,田素玉..气相色谱法同时测定多糖中的中性糖、糖醛酸、氨基糖和唾液酸[J].食品与发酵工业,2011,37(9):208-211,4.基金项目
河南省重点科技攻关项目 （）
