食品与发酵工业2011，Vol.37Issue(10)：92-95,4.
南极磷虾蛋白酶分离纯化及部分性质研究
Partial Purification and Characterization of Protease from Antarctic krill （Euphausia Superba）
摘要
Abstract
A protease that plays a role on autolysis physical and chemical characteristics were studied. The of Antarctic krill was purified from Antarctic krill and its major purified protease was obtained after 30% - 70% ammonium sulfate grading precipitation, DEAE-Sepharose Fast Flow anion-exchange chromatography and Sephacryl S -200 HR chromatography. The protease was confirmed by a single band on SDS-PAGE and was purified by 12.59-fold with the yield of 43%. The relative molecular mass of the enzyme was determined to be about 28 ku. The casein was used as substrate to measure the characteristics of the protease. The optimum pH and temperature of the enzyme were 8.0 and 50℃ , respectively. The enzyme was stable when pH value is in the range of 7.0 to 9.5 and the temperature is blow 45℃ ; PMSF （phenylmethyl sulfonyl fluoride）, SBTI （strontium bismuth titanate） and TLCK （N-α-Tosyl-L-Lysine Chloromethyl Ketone） can obviously inhibit the enzyme.关键词
南极磷虾/蛋白酶/分离纯化/性质Key words
Antarctic krill/protease/purification/characterization分类
轻工纺织引用本文复制引用
杭虞杰,李学英,杨宪时,迟海,郭全友..南极磷虾蛋白酶分离纯化及部分性质研究[J].食品与发酵工业,2011,37(10):92-95,4.基金项目
国家高技术研究发展计划项目 （）
中央级公益性科研院所基本科研业务费专项资金 （）
