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巴旦木蛋白水解物的制备及其抗氧化活性

孙月娥 王卫东 郑义

食品与发酵工业2011,Vol.37Issue(10):105-109,5.
食品与发酵工业2011,Vol.37Issue(10):105-109,5.

巴旦木蛋白水解物的制备及其抗氧化活性

Study on Preparation and Antioxidant Activity of Hydrolysate from Badanmu Protein

孙月娥 1王卫东 2郑义1

作者信息

  • 1. 徐州工程学院食品学院,江苏徐州221111
  • 2. 江苏省食品生物加工工程技术研究中心,江苏徐州221111
  • 折叠

摘要

Abstract

With the badanmu protein as the substrate, the orthogonal test was taken to study the enzymatic hydrolysis condition of alcalase enzyme on badanmu proteins. The reducing power and the scavenging activity to hydroxyl, superoxide radical, and DPPH · free radicals were also investigated. The optimum hydrolysis parameters were as follows : substrate concentration 20% , enzyme concentration 4% , pH value 9.5, temperature 60℃. In this condition of hydrolysis, its hydrolysis degree for badanmu protein is 36.6%. The reductive ability of badanmu protein peptide increased with peptide concentration increasing. The protein peptide also had good OH·, O2- , and DPPH · scavenging activity, which increased with the concentration of peptides until its peak. The antioxidation activity of badanmu peptides were almost fixed wheff its concentration exceeded a certain level.

关键词

碱性蛋白酶/巴旦木肽/抗氧化活性

Key words

alcalase enzyme/badanmu peptides/antioxidation activity

分类

轻工纺织

引用本文复制引用

孙月娥,王卫东,郑义..巴旦木蛋白水解物的制备及其抗氧化活性[J].食品与发酵工业,2011,37(10):105-109,5.

基金项目

苏北科技发展计划项目 ()

徐州工程学院培育项目 ()

食品与发酵工业

OA北大核心CSCDCSTPCD

0253-990X

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