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利用菊芋菊粉制备R,R-2,3-丁二醇发酵工艺条件的优化

杨欢欢 高健 徐虹 邵荣

食品与发酵工业2011,Vol.37Issue(11):6-10,5.
食品与发酵工业2011,Vol.37Issue(11):6-10,5.

利用菊芋菊粉制备R,R-2,3-丁二醇发酵工艺条件的优化

Optimization of Fermentation Conditions for R,R-2,3-butanediol Production Using Inulin Extract from Jerusalem artichoke tubers

杨欢欢 1高健 1徐虹 2邵荣1

作者信息

  • 1. 南京工业大学食品与轻工学院、材料化学工程国家重点实验室,江苏南京210009
  • 2. 盐城工学院化学与生物工程学院,江苏盐城224051
  • 折叠

摘要

Abstract

To increase the quality of R,R-2,3-Butanediol using inulin extract from Jerusalem artichoke tubers by Paenibacillus polymyxa ZJ-9 and decrease the costs of production,the fermentation conditions including cultural temperature, initial pH,inoculum concentration and agitation speed were optimized.The optimal conditions were confirmed as follows;cultural temperature 30℃,the optimization initial pH 6.0,inoculum concentration 8%(v/v).A two-stage agitation speed control strategy was proposed based on analyzing the microbiol physiological characteristics under different agitation speeds.During the first 24 h,agitation speed was controlled at 240 r/min to obtain high specific cell growth rate for cell growth,subsequently agitation speed was controlled at 160 r/min to maintain high specific R,R-2,3-BD formation rate for high R,R-2,3-BD accumulation.The concentration of the obtained R,R-2,3-BD was 27.83 g/L under this optimal conditions,increase 10.15%than that before optimization.

关键词

R,R-2,3-丁醇/多粘类芽孢杆菌/发酵工艺条件

Key words

R/R-2/3-butanediol/Paenibacillus polymyxa ZJ-9/fermentation conditions

分类

轻工纺织

引用本文复制引用

杨欢欢,高健,徐虹,邵荣..利用菊芋菊粉制备R,R-2,3-丁二醇发酵工艺条件的优化[J].食品与发酵工业,2011,37(11):6-10,5.

基金项目

江苏省自然科学基金计划项目 ()

江苏省教育厅高校科研成果产业化推进项目 ()

盐城工学院科研基金项目 ()

食品与发酵工业

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