食品与发酵工业2011，Vol.37Issue(11)：16-20,5.
酿酒酵母代谢木糖工程菌的构建
Construction of Engineering Strain Saccharomyces cerevisiae Metabolizing Xylose
摘要
Abstract
Xylose reductase and xylitol dehydrogenase from Candida shehatae amplified by PCR were ligated to yeast expression vector pYES2,to establish recombinant expression vectors pYES2-XYL1 and pYES2-XYL2,respectively. Recombinant expression vector pYES2-XYL1-XYL2 which was acquired by legating XYL1 harboring GAL1 promoter cloned from pYES2-XYL1 to pYES2-XYL2 downstream was transformed to Saccharomyces cerevisiae host strain INVSc1 by electroporation.The ethanol yield was 33.45 g/L under the conditions;initial pH5.5,temperature at 33℃,rotation speed at 50r/min after 150 r/min for 5 h.关键词
休哈塔假丝酵母/木糖还原酶/木糖醇脱氢酶/pYES2-XYL1-XYL2/酿酒酵母工程菌Key words
Candida shehatae/xylose reductase/xylitol dehydrogenase/pYES2-XYL1-XYL2/engineering strain Saccharomyces cerevisiae分类
轻工纺织引用本文复制引用
汤斌,张风琴,汤文晶,丁丽霞..酿酒酵母代谢木糖工程菌的构建[J].食品与发酵工业,2011,37(11):16-20,5.基金项目
安徽省高校自然科学基金重点资助项目 （）
