食品与发酵工业2011，Vol.37Issue(11)：21-25,5.
酸性条件下毛霉和根霉蛋白酶的催化特性
Characteristics of A.elegans and R.oligosporus Protease Under Acidic Condition
摘要
Abstract
Proteolysis of soybean protein isolated（SPI） by A.elegans and R.oligosporus proteases,which were cultured in their optimal enzyme production conditions,under acidic conditions pH3.0～6.0 was investigated.The results showed that,A.elegans and R.oligosporus proteases were able to quickly catalytic soybean 7s and 11s protein degradation when pH was 3.0～3.5,but at the moment,water - soluble protein yield were not high,all under 35%. At pH5.0,5.5 and 6.0,A.elegans and R.oligosporus protease all had stronger catalytic ability of degradation of SPI,both the hydrolysis degree and water-soluble protein increased,but the water - soluble protein yield from A.elegans had always been about 10%higher.SDS - PAGE showed the catalytic rate of A.elegans protease to soybean protein isolated reduced,and appeared with new bands,especially basic subunits was not able to be degraded in four hours.The speed of R.oligosporus protease catalytic degradation of soybean protein isolated was still stronger at pH5.0 and 5.5,but declined obviously at pH6.0,The acidic subunits and basic subunits were not degraded in four hours.In a word,there had big differences between the two proteases on catalysis characteristics at acidic condition.关键词
雅致放射毛霉/少孢根霉/酸性蛋白酶/大豆分离蛋白Key words
A.elegans/R.oligosporus/acidic proteolysis/soybean protein分类
轻工纺织引用本文复制引用
李晶晶,李理..酸性条件下毛霉和根霉蛋白酶的催化特性[J].食品与发酵工业,2011,37(11):21-25,5.基金项目
国家自然科学基金资助项目（30770056）。 （）
